Course description
The course will deal with different aspects of modern basic and advanced cellular and molecular imaging.
This course provides:
- Basic concepts in modern fluorescence microscopy: What is fluorescence, different fluorochromes, excitation methods, emission characteristics, purpose and uses
- Basic concepts in modern transmission and scanning electron microscopy.
- How to prepare cells/tissues for light and electron microscopy.
- Advanced laser systems: Laser Scanning confocal microscope: Fundamentals of confocal microscopy, advantages in comparison to fluorescence microscopy, different approaches and instrumentation limitations. Will include line scanning microscopy, spinning disk microscopy, multicolour, Z-stacks, user tips.
- Functional microscopy: FRET, FRAP, FCS, FCCS, etc.
- Advanced techniques in electron microscopy: Correlative light and electron microscopy (CLEM), 3D electron-tomography; 3D array-tomography; High pressure freezing and freeze substitution; Tokuyasu method, etc.
- Application of advanced microscopy methods for current research topics
- Multiphoton confocal microscopy
- Super-resolution microscopy: single molecule localization and structured illumination
- Laser capture microdissection for non-contact sample handling
- High content screening fluorescence microscopy for system biology
Teaching will be performed through lectures, group discussions, hands-on experiments, individual training and demonstrations with the microscopes, individual reading and writing a report.
Requirements and Selection
Entry requirements
To qualify for admission to the course, the student has to be registered as a doctoral student (third cycle) at Sahlgrenska Academy or at another faculty or university.
Selection
Selection is performed according to the following:
- PhD students at Sahlgrenska Academy,
- PhD students enrolled at another university within a formal collaborative agreement on doctoral education with Sahlgrenska Academy,
- PhD students enrolled at another faculty at the University of Gothenburg or another university in Sweden,
- PhD students enrolled at a university outside Sweden.
Other information
Course leader: Rafael Camacho, rafael.camacho@gu.se
Course administrator: Zerif Olsen, zerif.olsen@gu.se
Course dates: 23 March - 2 April 2026
- 23 - 27 March: lectures and demos,
- 30 March - 2 April: self-study and examination report preparation.
Course syllabus
SC00004
Department
Core Facilities at Sahlgrenska Academy
Subject
Health Sciences
Type of course
Method course
Keywords
cellulär avbildning, imaging